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RNA extraction and RT-PCR
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Nucleic acid extraction from nasopharyngeal samples was performed using the Extracta Kit—Viral RNA and DNA (MVXA‐ P016FAST) (Loccus, Brazil) using an Extracta32 instrument (Loccus) as previously described (15). Laboratory diagnosis was based on one-step reverse transcription followed by RT-PCR using the INFA/INFB/SC2 kit (Bio-Manguinhos, Brazil) as described previously (15).
Copyright and License information: ©2023 Pires Farias et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
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