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Cells were seeded at a density of 2 × 106 /well in a 6-well plate, and incubated until 100% confluence. The layer of cells was scratched with a 200 µL pipette tip (Sigma), and washed three times with PBS and incubated with fresh serum-free DMEM medium for 24 h. Finally, pictures were acquired with an inverted microscope (Nikon, Japan) and the gap coverage was acquired by statistical analysis.
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