Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Amyloid quantification and labeling
Request a Protocol
Ask a
question
Favorite
PSMα assemblies were prepared as described above, incubated in DIW for two hours at concentration of 440 µM and then buffered with Hepes(final PSMα concentration 400 µM, Hepes 50 mM). Amytracker-680 was diluted with DIW by 50. 55 µL of PSMα solution was mixed with 5 µL of Amytracker-680 stock solution (Amytracker-680 final dilution was *500). Then, the samples were placed in 384 well black plate, incubated for 10 min and then fluorescent measurement was applied (Excitation at 550 nm, emission 650 nm) at Biotek Synergy H1 plate reader (Biotek, Winooski, VT, USA).
Copyright and License information: The Author(s) ©2023
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
