Preparation of thermo-sensitive Poloxamer 407 (P407) System

Injectable thermosensitive P407 was used as a testosterone carrier for sustained-release drug delivery. The P407 powder was dissolved at a concentration of 21% in phosphate-buffered saline (PBS) and Ethanol with a volume ratio of 80/20. Then, a testosterone injection solution (ALL-TES-2433) was added to the P407 gel at a 5 gr/ml ratio. This solution was used in the early stages of the study to induce alopecia in animals. Furthermore, P407 maintained the slow release of umbilical cord MSC-CM (UC-MSc-CM). To achieve this, a suitable amount (200 μg) of dried CM was dissolved in P407 gel without ethanol to reach a concentration of 22% at 15°C (12, 18).