The chromosome location of HvJRLs was mapped based on their specific positions in the barley genome by TBtools v.1.120 [64]. The gene duplications of HvJRLs were determined by MCScanX (http://chibba.pgml.uga.edu/mcscan2/#tm (accessed on 17 August 2023)) [47]. If the alignment sequence covers more than 70% of the longer gene and the sequence identity is greater than 70%, it was defined as a gene duplication event [67]. The collinearity relationships were visualized using TBtools v.1.120 [64].
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.