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Detection and mapping the position of ISAbaI
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Presence of the insertion sequence, ISAbaI was investigated in all the isolates as described previously [18]. In order to map the position of ISAbaI in relation to bla OXA-51 and bla OXA-23 genes, two independent PCRs were carried out using the following combinations of primer pairs: (a) ISAbaI-forward primer (ISAbaIF) and bla OXA-51-like reverse primer (OXA-51-likeR); and (b) ISAbaIF and bla OXA-23-like reverse primer (OXA-23-likeR) [19].
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