In vivo experiments

The Animal Care Committee of Chongqing Medical University approved the animal experiments, which were carried out in accordance with the ethics Committee of Chongqing Medical University guidelines and regulations for animal welfare. Hunan SJA Laboratory Animal Co. Ltd. (Hunan, China) provided the female BALB/c naked mice (5 wk old). 1 × 10⁷ cells were injected subcutaneously into the shoulders of mice (n = five per group). Tumor growth was monitored every 3 d for 4 wk. To determine the tumor volumes, the formula v (mm³) = a × b × c/2 was used, where a is length, b is width, and c is height. IHC tests were performed using tumor samples.