Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
LPS- and OGD-treated cells were washed with PBS and stained with 5 μM 2′,7′-dichlorofluorescin diacetate (DCFH-DA; Sigma-Aldrich) for 20 min at 37°C to detect cell reactive oxygen species (ROS). After staining, cells were washed three times with PBS, and were detached with trypsin for flow cytometry, DCF fluorescence was detected at λex of 488 nm and λem of 525 nm.
Copyright and License information: ©2023 Gómez-Ferrer, Amaro-Prellezo, Albiach-Delgado, Ten-Domenech, Kuligowski and Sepúlveda
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
