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IHC quantification

WH Wouter S Hoogenboom
TR Todd G Rubin
KA Kamalakar Ambadipudi
MC Min-Hui Cui
KY Kenny Ye
HF Henry Foster
EE Esther Elkouby
JL Jinyuan Liu
CB Craig A Branch
ML Michael L Lipton
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An automated stain-intensity-based IHC quantification tool was used to analyse the stained sections (CaseViewer, 3DHISTECH Ltd., Budapest, Hungary) (Supplementary Fig. 6). Digitized images were used to segment the CC (CC1 = genu, CC2 = anterior mid-body, CC3 = mid-body, CC4 = posterior mid-body, CC5 = splenium). Additionally, five circular ROIs (1.2 mm2) were placed in the cerebral white matter (CTX) halfway between the CC and cortical surface.

Copyright and License information:  ©2023 The Author(s) 2023. Published by Oxford University Press on behalf of the Guarantors of Brain.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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