Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Cell viability assay

AN Almar Neiteler
AP Anwar A. Palakkan
KG Kevin M. Gallagher
JR James A. Ross
request Request a Protocol
ask Ask a question
Favorite

RPE cell lines were seeded at 104 cells per well in a 96-well plate. After 24 h, cells were then treated with 500 µM H2O2 or 500 µM BSA-PA or 500 µM BSA-DHA or H2O2 with BSA-PA or H2O2 with BSA-DHA for 6 or 18 h. Respective wells were pre-treated with 40 µM Nec-1 or 5 µM Fer-1 for 1 h before the treatment. Cells treated with 0.67% BSA and 0.17% ethanol were considered as the control (vector control). After treatment cellular viability was analysed using MTT. Briefly, cells were incubated with 0.5 mg/ml MTT (Sigma-Aldrich) for 4 h, and the formed formazan crystals were solubilised using 10% SDS-(0.01N)HCl solution. Absorbance at 570 nm and 690 nm was measured, and the 570/690 ratio was used for the comparison. The absorbance ratio of each group was compared to the control and expressed as a percentage.

Copyright and License information: The Author(s) ©2023
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A