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Immunohistochemistry (IHC) and immunofluorescence (IF) procedures
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In this investigation, we focused on IHC and IF analyses for NF-L and histone-H3 only in those brain regions where these proteins were found to be differently expressed in RAD versus SH animals based on WB results. Specifically, we performed IHC for NF-L and histone-H3 in the CRB, FCtx and Str, and IF for histone-H3 only in the FCtx and Str.
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