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CellTiter-Glo 3D Cell Viability Assay (Promega, cat# G9681) was used according to the manufacturer’s instructions. CellTiter-Glo 3D Reagent was thawed overnight at 4 °C and brought to room temperature (RT) for 20-min before use. After the FLIPR assay, 30 µL of CellTiter-Glo 3D Reagent was added to the spheroid plate and was mixed by shaking for 5-min at RT followed by a 25-min incubation period on the shaker at RT. Luminescence was read using a PHERAstar FSX microplate reader (BMG LabTech) to measure the amount of adenosine 5′-triphosphate (ATP) present.
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