The response to tumor growth was monitored using bioluminescence imaging with an IVIS spectral imaging system (PerkinElmer, Waltham, MA). Bioluminescence IVIS acquisitions were carried out throughout the duration of the experiment. To facilitate imaging, mice were treated with D-luciferin (150 mg/kg) via intraperitoneal injection. Mice were anesthetized using gas anesthesia with 2.5% isoflurane 15 min after luciferin administration. Imaging was conducted on black paper within the IVIS imaging system box.
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