Induction of the rat myocardial infarction model and treatment with ITE

This animal experiment was approved by the Laboratory Animal Ethics Committee of Fujian Medical University. Eight‐week‐old male Sprague–Dawley rats (body weight of 200 ± 10 g) were purchased from Shanghai Slack Company. The MI model was established by permanent ligation of the left anterior descending (LAD) coronary artery under ventilator support. ¹⁴ Rats that survived after surgery were verified by cardiac ultrasound after 1 week and randomly divided into the MI group (MI, n = 8), MI with 1.6 mg/kg/day ITE treatment group (MI + ITE1.6, n = 8), and MI with 8 mg/kg/day ITE treatment group (MI + ITE8.0, n = 8). The high and low doses of ITE used in this study were described in the literature. ¹⁵ In addition, the control group (control, n = 8), sham group (sham, n = 8), control + ITE1.6 group (n = 8), and control + ITE8.0 group (n = 8) were set up; dimethyl sulfoxide (DMSO) (Sigma‐Aldrich, St. Louis, MO, USA), PEG300, Tween 80 (VICMED Company), and normal saline were added to ITE (Eisai Company) at a ratio of 1:3.5:1:9.5, mixed well, and administered by intraperitoneal injection once a day.