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Plasma retrieval and analyses

AK Anki Knutsson
SH Sabrina Hsiung
SC Selvi Celik
SR Sara Rattik
IM Ingrid Yao Mattisson
MW Maria Wigren
HS Howard I. Scher
JN Jan Nilsson
AH Anna Hultgårdh-Nilsson
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Blood was collected by cardiac puncture and plasma retrieved by centrifugation at 3000 rpm for 15 min at 4 °C. The colorimetric assay Infinity Total Cholesterol (Thermo Scientific, Liverpool, UK) was used to quantify total plasma cholesterol and the Bio-Plex Pro Mouse Cytokine Assay (BIO-RAD) was used to quantify plasma cytokine concentrations of with IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-13, IL-17A and tumor necrosis factor-α (TNF-α). Both analyses were performed according to instructions from the manufacturer.

Copyright and License information:  ©2016, Macmillan Publishers Limited
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