2.8. Detection of cell antioxidant capacity by SOD, GSH-Px, CAT, MDA and T-AOC

After grouping and incubating the cultured CEF cells with toxins, the culture medium is collected in centrifuge tubes at the desired time. The cells are washed twice with pre-chilled PBS at 4 °C, and the cells adhered to the walls are scraped off using a cell scraper and collected in the centrifuge tubes. The tubes are then centrifuged at 400 g for 10 min, and the supernatant is discarded. Please note that all steps should be performed on ice or at 4 °C. Then determine the concentration of total protein using the BCA assay kit. Levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), malondialdehyde (MDA) and total antioxidants (T-AOC) were determined spectrophotometrically according to the instructions provided in the assay kit.