Cell culture

SS Saimeng Shi
DG Duancheng Guo
LY Longyun Ye
TL Tianjiao Li
QF Qinglin Fei
ML Mengxiong Lin
XY Xianjun Yu
KJ Kaizhou Jin
WW Weiding Wu
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HEK293T cells, H6C7 cells and human pancreatic cancer cell lines (SW1990, BxPC-3, Mia-PaCa-2, Capan-1, CFPAC-1, and Panc-1) were purchased from Cell Bank, Type Culture Collection, Chinese Academy of Sciences. All human cell lines have been authenticated using STR profiling and tested with no mycoplasma contamination. The cells were cultured in a humidified incubator with 37 °C, 5% carbon dioxide. H6C7 and BxPC-3 were cultured in RPMI-1640 with 10% FBS; Capan-1 and CFPAC-1 were cultured in IMDM with 10% FBS; and other cells were cultured in high glucose DMEM with 10% FBS.

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