2.3. Virus Stock Preparation

RM R. K. Subbarao Malireddi
BS Bhesh Raj Sharma
RB Ratnakar R. Bynigeri
YW Yaqiu Wang
JL Jianlin Lu
TK Thirumala-Devi Kanneganti
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The IAV (A/Puerto Rico/8/34, H1N1 [PR8]) was prepared as previously described [12] and propagated from 11-d-old embryonated chicken eggs by allantoic inoculation. The IAV titer was measured by plaque assay in Madin–Darby canine kidney (MDCK) cells.

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