3.2.2. Glycosylation (Standard Procedure B)

A mixture of a glycosyl acceptor [0.16 mmol] and a glycosyl donor [0.16 mmol, 1 eq.] was co-evaporated with toluene twice and dried for 1 h in vacuo, and then the flask was filled with Ar. Freshly activated molecular sieves 4 Å [100 mg] and anhydrous DCM [2 mL] were added and the mixture was stirred for 2 h at rt. Then, the solution of TMSOTf in DCM [10% v/v, 60 μL, 0.2 eq.] was added at −50 °C under argon protection. The mixture was stirred under argon protection until TLC showed full consumption of the starting materials, and then neutralized with Et₃N and the mixture was purified by chromatography (silica gel, eluent: hexane/EtOAc) to give a glycosylation product as a mixture of α- and β-isomers.