The emulsification index was determined using the method described by Cooper and Goldenberg [19], where 2 mL of a hydrocarbon was added to 2 mL of the cell-free metabolic liquid in an assay tube and vortexed for 2 min. Emulsion stability was determined after 24 h and the emulsification index was calculated by dividing the height of the emulsion by the total height of the mixture and multiplying by 100.
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