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2.5. Determination of Enzyme Activity and Secondary Metabolites

TH Ting Huang
YZ Yi Zhang
ZY Zhihe Yu
WZ Wenying Zhuang
ZZ Zhaoqing Zeng
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Protease, cellulase, and β-1,3-glucanase were detected on skim milk, carboxymethyl cellulose (CMC), and glucose agar, respectively [29]. Siderophore and indole-3-acetic acid (IAA) were tested using modified chrome azurol S and Salkowski reagent agar, respectively [30]. Enzyme activity and production of metabolites were observed based on the presence of clear zones around bacterial colonies after incubation at 28 °C for 3 d. All treatments were repeated three times.

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