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The pGMPPAR-Lu reporter plasmid was obtained from Genomeditech Co., Ltd. (Shanghai, China). Human embryonic kidney (HEK) 293 cells were seeded on a 24-well plate and transfected with 450 ng of pGMPPAR-Lu and 50 ng of pRL-TK per well. After 24 h of transfection, the cells were treated with EPA-PC/PE/PS and DHA-PC/PE/PS (40, 60, or 80 µg/mL) for 24 h. Following cell lysis, luciferase activities were analyzed using Dual-Luciferase Reporter Assay System (Promega, Madison, WI, USA) in accordance with the manufacturer’s recommended procedure.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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