3.4.2. Preparation of the Spinning Solutions and Electrospinning

All spinning solutions were prepared under stirring for 24 h to ensure their homogeneity. The spinning solution of PCL was prepared at room temperature, whereas all the aqueous spinning solutions were prepared at 60 °C. For the preparation of the PCL spinning solution, PCL was dissolved at a concentration of 10% w/v in DCM/DMF 8:2 v/v. The CG-Ca/PEO and the CG-Na/PEO spinning solutions were prepared by dissolving CG-Ca or CG-Na, respectively, at a concentration of 1% w/v in distilled H₂O followed by the addition of PEO (Mw 900,000) at a concentration of 4% w/v. The PG-Ca/PEO spinning solution was prepared by dissolving PG-Ca at a concentration of 4% w/v in distilled H₂O followed by the addition of PEO (Mw 8,000,000) at a concentration of 4% w/v. The CG-Ca/PG-Ca/PEO spinning solution was prepared by dissolving CG-Ca at a concentration of 1% w/v in distilled H₂O followed by the addition of PG-Ca at a concentration of 4% w/v and PEO (Mw 8,000,000) at a concentration of 4% w/v.

Electrospinning was conducted using a γ-High Voltage Research DC power supply generator of 50 kV maximum voltage (Gamma High Voltage Research, Ormond Beach, FL, USA). The spinning solutions were loaded into 10 mL disposable syringes fitted with stainless steel blunt needles (23G). The syringes were mounted on a horizontally positioned programmable syringe pump (Harvard PHD 2000, Harvard Apparatus, Holliston, MA, USA) and the produced nanofibers were deposited on a RC-6000 drum collector (NaBond Technologies, Hong Kong) rotating at 400 rpm. Temperature and relative humidity were 22 ± 2 °C and 65 ± 5%, respectively.