2.13. Statistical Analysis

The SPSS statistical software package 20.0.0 (SPSS Inc., Chicago, IL, USA) and GraphPad Prism 6.0 (GraphPad Software, Inc., La Jolla, CA, USA) for Macintosh were used for all statistical analyses and graph design. Data are mean values ± standard deviation of the mean (SD). All the experiments were performed with 8 mice (n = 8) per experimental group. The normality of the data was analyzed using the Kolmogorov–Smirnov test. Since all data had normal distribution, they were analyzed with a parametric two-way ANOVA followed by Bonferroni post hoc test. A p < 0.05 was considered statistically significant.