Quantification of gene expression by real-time PCR

KM Katia Mangano
MP Maria Cristina Petralia
RB Rita Bella
MP Manuela Pennisi
JM José Francisco Muñoz-Valle
JH Jorge Hernández-Bello
FN Ferdinando Nicoletti
PF Paolo Fagone
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Primer sequences were designed in-house or downloaded from PrimerBank (https://pga.mgh.harvard.edu/primerbank/). Sequences for primers are listed in Table Table1.1. Real-time PCR was performed using the QuantiNova SYBR Green mastermix (Qiagen). Gene expression was calculated using the formula: 2−ΔΔCt, where ΔΔCt = (Cttarget gene − CtGAPDH) EAE – (Cttarget gene − CtGAPDH)control.

Primers used in this study

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