Primer sequences were designed in-house or downloaded from PrimerBank (https://pga.mgh.harvard.edu/primerbank/). Sequences for primers are listed in Table Table1.1. Real-time PCR was performed using the QuantiNova SYBR Green mastermix (Qiagen). Gene expression was calculated using the formula: 2−ΔΔCt, where ΔΔCt = (Cttarget gene − CtGAPDH) EAE – (Cttarget gene − CtGAPDH)control.
Primers used in this study
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