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OCI-Ly8 was transduced to express green click beetle luciferase and ZsGreen using pHIV-Luc-ZsGreen (a gift from Bryan Welm [Addgene plasmid no. 39196]). These cells were cultured with healthy donor T cells (E:T ratio of 5:1) and different concentrations of mosunetuzumab (Genentech and Roche): 0, 1, 10, and 100 ng/ml. After 24 hours, cancer cell survival was monitored by luciferase bioluminescence using a BioTek Synergy H4 Imager (560 nm). Cell viability (%) was calculated relative to that of the Ctrl cancer cells and T cells without mosunetuzumab.
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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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