Computational epitope prediction of serum IgG-neutralizing activity was conducted as described previously61,80. In brief, the neutralizing IC50s of the respective participant’s IgGs were determined against the 20 pseudovirus f61 panel. Following, the similarity of the fingerprint of the tested participants’ IgGs is compared to the fingerprint of ten classes of reference-bNAbs, grouped by their specific epitope; for each class, shown is a prototypic bNAb member. The prevalence of these reference antibody epitopes in a respective participant’s IgG is computationally predicted and assigned a delineation score between 0 (low) and 1 (high). The scores as shown in Fig. Fig.2a2a represent the percentage ((initial delineation score) × 100).
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