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Antioxidant activity of SeO32− and SeNPs selenite using the DPPH assay

JT Job T. Tendenedzai
EC Evans M. N. Chirwa
HB Hendrik G. Brink
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The antioxidant potency of SeO32−, abiotic-SeNPs, and chemical-SeNPs was measured using DPPH (2,2-diphenyl-2-picrylhydrazyl hydrate) analysis. This is a DPPH radical scavenging assay similar to the one published elsewhere32. 5 ppm, 10 ppm, 20 ppm, 40 ppm, and 80ppm of either SeO32−, abiotic-SeNPs, and chemical-SeNPs were used. The solutions (prepared in DMSO) to be investigated were mixed with 2 mL of a 0.2 mM solution of DPPH in a methanol solvent and mixed well. Thereafter, the mixture was incubated for 30 min in the dark. The absorption of the samples was detected at 517 nm using a UV–vis. L-ascorbic acid (C6H8O6) was used as the standard. The equation for measuring antioxidant activity, similar to the one described by Al Jahdaly et al.33 is indicated below;

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