Rota-rod test was performed to evaluate the effect of fasting on motor performance. All mice were trained to walk on a rota-rod apparatus (SciTech Korea Inc., Seoul, Korea) for 3 days before the initiation of experiments. Each mouse was placed on a rotating rod at a speed of 10 rpm for 5 min and the walking time (sec) was measured. After 15-min break, the test was performed again and repeated for a total of 120 min.
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