Ziehl–Neelsen (ZN) staining was performed to all clinical specimens (4733). In addition, molecular methods were performed among 3139 clinical specimens, according to manufacturer's instructions. Testing was performed whenever requested by the clinicians. COBAS AMPLICOR MTB PCR (Roche Diagnostics, Rotkreuz, Switzerland) was used in 814 specimens obtained during January to September of 2009, whereas, COBAS TaqMan MTB (Roche Diagnostics) was applied afterwards in 2325 specimens, thereafter (October 2009–December 2011).
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