2.10. Intramacrophage Assay

The intramacrophage assay was performed following the method proposed by Snewin et al. [35]. Macrophages were infected with MTB, and after internalization extracellular mycobacteria were removed with amikacin as well as antibiotic residues by washing with PBS. Infected macrophages were treated with the nanoliposomes at 1×, 4× and 10× MIC values. Macrophages were lysed after 72 h of infection with triton X-100 (0.01%) and plated on 7H11 agar plates supplemented for 25 days under SC. The results were expressed in CFU/mL and compared with the growth obtained from untreated wells.