2.11. Detection of cisplatin accumulation

For the detection of renal cisplatin accumulation, mice were intraperitoneally injected with 20 mg/kg CP 30 min after 25 mg/kg vaccarin or NS administration. The mice were euthanized 6 h after cisplatin injection and the renal blood was drained by cardiac perfusion with NS. The renal cortex tissues were incised, weighted, and then digested with 3 % nitric acid for platinum (Pt) analysis by ICP-MS, according to our previous study [11].