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Cytospins and Wright–Giemsa Staining
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Cells from BALF were spun onto glass slides by a cytospin. After the slide was mixed in methyl alcohol, Wright–Giemsa A was added onto it for 1 min, and then Wright–Giemsa B was added on top of Wright–Giemsa A for 5 min. The dye liquor was washed off gently by running water, followed by drying and observation under a microscope. To count differential inflammatory cell number, 300 cells in total were counted, and 100 of the cells in each microscopic field were scored. The mean number of cells per field was reported.
Copyright and License information: ©2017 Liu, Lv, Wen, Ci and Peng.
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