L-Phenylalanine ammonia-lyase enzyme activity (PAL)

SS Saba Samadi
MS Mohammad Jamal Saharkhiz
MA Majid Azizi
LS Leila Samiei
MG Mansour Ghorbanpour
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Activity of PAL (unit g−1 FW) was studied using a method that involved a spectrophotometer at 290 nm [33]. Briefly, the enzyme reaction mixture (1 ml) comprised of L-phenylalanine (0.5 ml, 10 mM), enzyme extract (0.1 ml) and 0.05 M Tris–HCl buffer (pH 8.0). The reactions were stopped by 1 N Hydrogen chloride (0.1 ml).

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