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Histological analysis

LZ Long-mei Zhao
LD Lin-cui Da
RW Rui Wang
LW Long Wang
YJ Yan-lin Jiang
XZ Xiu-zhen Zhang
YL Ya-xing Li
XL Xiong-xin Lei
YS Yu-ting Song
CZ Chen-yu Zou
LH Li-ping Huang
WZ Wen-qian Zhang
QZ Qing-yi Zhang
QL Qian-jin Li
RN Rong Nie
YZ Yi Zhang
YL Yan Liang
JL Jesse Li-Ling
HX Hui-qi Xie
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At 4, 8, and 12 weeks after the surgery, the animals were euthanized. The operative area of each uterine horn was prepared and subjected to H&E and Masson’s trichrome staining. The thickness of the uterine endometrium was measured using ImageJ software.

For immunofluorescence staining, serial sections were immunolabeled with anti–EpCAM (1:200; ab221552, Abcam), Lgr5 (1:200; ab219107, Abcam), Ki67 (1:200; ab15580, Abcam), anti-α-SMA (1:200; ab7817, Abcam), anti-myosin (1:100; ab182981, Abcam), and FoxA2 (1:200; ab108422, Abcam). ImageJ software was used for analyzing the images, including the percentage of positive stained areas and glands. The percentage of glands was calculated with the following Eq. 2

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This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.

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