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Sample size was calculated based on pilot feasibility data with 5 participants put through all 3 phases. We calculated sample size using changes in fasted insulin and IGF-1 concentrations. The sample size was estimated using G*Power (v3.1) with an alpha level of 0.05, a power (1-β) of 0.80, a medium effect size of f = 0.5, and a conservative intra-measurement correlation of 0.5. This analysis recommended a sample size of n = 9, that predicted to produce results with an effect size of 1.1 dz for paired comparisons.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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