Mice (female, 6–8 weeks old, n = 8) were exposed to CS for 8 weeks, control mice were exposed to normal air. Some mice were treated with 50 mg/kg disodium cromoglycate (DSCG, cat# 15826-37-6, Sigma) intranasally from weeks 6 to 8 of CS exposure, and control mice received equal volumes of vehicle. BALF was collected for assessment of inflammatory cell influx, lungs were perfused, formalin-fixed, paraffin-embedded and sectioned (3.5 μm thickness) for histology, stained with H&E for emphysema analysis, and lung function was assessed using the flexiVent system27,28,42,46. Proteins were extracted from mouse lungs and mMCP6 levels were measured by immunoblot41.
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