Adipocyte differentiation

SP Se-Ra Park
SK Soo-Rim Kim
EM Eun-Kyung Min
BO Byung-Chul Oh
YJ YunJae Jung
YK Yong Ho Kim
HL Hwa-Yong Lee
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Human or mouse endometrial stem cells were cultured in low-glucose DMEM supplemented with 500 µM methylxanthine, 5 µg/mL insulin, and 10% FBS for 3 weeks with medium change twice per week. The formation of lipid droplets was confirmed by oil red O staining, and their abundance was measured by calculating the absorbance at 500 nm.

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