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Ladder rung walking test

KŠ Kateřina Štepánková
MC Milada Chudíčková
ZŠ Zuzana Šimková
NM Noelia Martinez-Varea
ŠK Šárka Kubinová
LU Lucia Machová Urdzíková
PJ Pavla Jendelová
JK Jessica C. F. Kwok
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For the advanced locomotor skills, the ladder rung walking test had been used. Animals were placed on a 1.2 m—long horizontal ladder, with irregularly spaced rungs. At the end of the ladder was a dark box that the rats favoured. The animals crossed the ladder 3 times in a row and all attempts were recorded on camera. The hind paws placement on the rungs was evaluated by using a seven-category scale (0–6 points), as previously described by Metz and Whishaw86 from all three videos. Metz and Whishaw´s scoring scale was divided into 3 categories: 0–2; 3–4; 5–6. Videos were then evaluated and the percentage of steps in each of the 3 categories was calculated. All the animals were pretrained before lesioning. The test was performed weekly for 16 weeks, starting 6 weeks after the SCI.

Copyright and License information: The Author(s) ©2023, corrected publication ©2024
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