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To test muscle strength, mice were placed in the center of a stainless-steel cooling rack for baking (20.32 cm × 29.85 cm; grid spacing: 1.27 cm square). The rack was inverted and held 46 cm above a cage containing bedding. The time mice could remain suspended was measured; 60 s was the cut-off time to prevent exhaustion. Each mouse was tested 5 consecutive times and the average time of staying suspended (latency to fall) was calculated.
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