Firefly Luciferase (fLuc+) RMS cell lines

AT Andrea Timpanaro
CP Caroline Piccand
DD Dzhangar Dzhumashev
SA Stenija Anton-Joseph
AR Andrea Robbi
JM Janine Moser
JR Jochen Rössler
MB Michele Bernasconi
ask Ask a question
Favorite

pHIV-iRFP720-E2A-Luc was a gift from Antonius Plagge (Addgene plasmid #104587) and was used to generate iRFP720+fLuc+ lentiviruses and to stably transduce RD, Rh4, and JR cell lines at an MOI of 5. iRFP720+fLuc+ RMS cell lines were sorted 48 h after transduction and amplified.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A