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Salicylic acid (SA) was dissolved in the deionized water and added to the YPD medium with the final concentrations of 0.25 mg/L, 0.5 mg/L, 1 mg/L, 2 mg/L and 3 mg/L, respectively (SA-treatment). Similar amounts of deionized water were added to the YPD medium and used as the controls (SA-free). To select the positive transformants overexpressing the PT gene, YM medium plate containing 50 mg/L G418 was prepared on which transformants were cultured.
Copyright and License information: ©2023 Jia, Li, Luan, Liu, Chen and Bao.
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