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MiRNA targets were predicted using TargetScan and miRDB. The cells (5 × 104) were seeded into 24-well plates and cultured for 24 h. The reporter luciferase plasmid (100 ng), pGL3, pGL3–mut or control luciferase plasmid, and 5 ng pRL-TK Renilla plasmid (Promega, Madison, WI, USA) were transfected into the cells using Lipofectamine 3000 according to the manufacturer’s instructions. Luciferase and Renilla signals were measured 48 h after transfection using a Dual-Luciferase Reporter Assay Kit (Promega) according to the manufacturer’s protocol.
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