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Endothelial permeability assay in vitro

YL Yiwei Lu
GH Guoyong Han
YZ Yao Zhang
LZ Long Zhang
ZL Zhi Li
QW Qingyuan Wang
ZC Zhiqiang Chen
XW Xuehao Wang
JW Jindao Wu
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Endothelial permeability assay was performed according to a previous study [16]. Briefly, 2 × 104 HUVEC cells were seeded into 24-well Transwell filters (8-μm pore size; Corning) and then incubated when cell confluency reached 100%. Afterward, the 24-well Transwell filters were carefully washed with PBS. Finally, 20 mg/mL rhodamine-dextran (average MW: ~ 70,000; Sigma-Aldrich) was added into the upper chamber, and after 1 h of incubation, the lower medium was collected and assessed under laser confocal scanning microscopy.

Copyright and License information: The Author(s) ©2023
Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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