Examining serum glucose and insulin levels

To examine glucose and insulin tolerance, the mice were intraperitoneally injected with glucose at a dose of 2.0 g/kg and insulin at a dose of 0.75 U/kg on GD10 after a 6-h fasting period. The glucose levels were measured at 0 (as baseline), 30, 60, and 120 min after the injection using a glucose meter (Roche Diagnostics, Switzerland). In addition, the serum glucose and insulin levels were measured using a glucose meter and a mouse insulin ELISA kit (Ultrasensitive) (catalog number: PI602, Beyotime, Shanghai, China) according to the manufacturer’s instruction, respectively.