PLC/PRF/5 sphere cells were seeded in 6-well culture plates and then infected with ZD55 or GD55 at the indicated MOIs; uninfected cells served as control. 48 h later, cells were stained with Hoechst 33342 (Beyotime, shanghai, China) at 1 mg/ml for 30 min at 37°C and observed under the inverted fluorescence microscope.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.