Hoechst 33342 staining

XZ Xinmin Zhang
SM Shulin Meng
RZ Rong Zhang
BM Buyun Ma
TL Tao Liu
YY Yu Yang
WX Wenjie Xie
XL Xianglei Liu
FH Fang Huang
TL Tao Liu
XZ Xiumei Zhou
XL Xinyuan Liu
YW Yigang Wang
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PLC/PRF/5 sphere cells were seeded in 6-well culture plates and then infected with ZD55 or GD55 at the indicated MOIs; uninfected cells served as control. 48 h later, cells were stained with Hoechst 33342 (Beyotime, shanghai, China) at 1 mg/ml for 30 min at 37°C and observed under the inverted fluorescence microscope.

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