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Screening for antibacterial activity

MD Mohammad Sadegh Damavandi
HS Hasan Shojaei
BE Bahram Nasr Esfahani
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Antimicrobial activity of the bioactive secondary metabolites was studied by agar well diffusion method against Staphylococcus aureus ATCC 25,923, Methicillin-resistant S. aureus ATCC 33,592, Enterococcus faecium ATCC 51,299, Salmonella enterica ATCC 14,028, Pseudomonas aeruginosa (P. aeruginosa) 27,852, Acinetobacter baumannii ATCC 19,606 at the concertation 10 mg/mL. Antimicrobial activity was detected by measuring the zone of inhibition appeared after the incubation period. A concentration of 10% DMSO was used as a negative control, while cefoxitin, oxacillin, and gentamicin antibiotic discs were used as positive controls.

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