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Preparation of Ehrlich Ascites Carcinoma (EAC) cells and induction of tumor in mice
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EAC cells were withdrawn from the donor mice and resuspended in sterile phosphate buffered saline (PBS). Viable cells (1 × 106 cells/22 g b. wt) were injected into the peritoneal cavity of each recipient mouse and allowed to multiply. The tumor cells were withdrawn after 8–10 days of inoculation, diluted in saline, counted and injected (1 × 106 cells/animal) in left thigh of animals for developing solid tumor.
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