TEM analysis of the liposomes was conducted in order to confirm their size and shape. A volume of 5 µL of the liposome dispersion was applied on Formvar film-coated copper grids. Measurements were performed using a JEOL JEM 2100 HT (Jeol Ltd., Tokyo, Japan) transmission electron microscope under the accelerating voltage of 80 kV. Images of liposomes were taken using a 4 k × 4 k camera (TVIPS) equipped with EMMENU software ver. 4.0.9.87 (TVIPS GmbH, Gauting, Germany).
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