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siRNA (negative control, LRRC8A, Nox1, p22phox, JNK1) were purchased from Dharmacon (Lafayette, CO). siRNA (100 nM) was incubated with Lipofectamine 2000 (Life Technologies) in serum-free medium for 20 min. The resultant complex of siRNA-Lipofectamine 2000 was added to cells in DMEM containing 5% FBS and then maintained for 3 days before performing experiments.
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